Bertarelli-funded Research Projects

PIs: Konstantina Stankovic, Harvard Medical School and Massachusetts Eye and Ear

Demetri Psaltis, École Polytechnique Fédérale de Lausanne

Title: Developing new methods for diagnostics of hearing loss
Project Summary:
One of the great challenges in diagnosing hearing problems is that the physician cannot see the tissues and cells of the inner ear. In contrast, simple optical methods allow inspection of the retina of the eye. In this continuation proposal the researchers will collaborate to develop new imaging methods for the human inner ear. While they have previously showed that they can image the inner ear with minimal invasion, they will now extend advanced endoscopic two‐photon technology to allow subcellular imaging, they will use the fluorescence of two natural metabolic products to assess the health of the inner ear, and they will extend initial results to enable imaging of the whole hearing organ. These experiments draw on the highly complementary skills of the two investigators to develop new methods for diagnostics for hearing loss. 

PIs: Daniel J. Lee and M. Christian Brown, Harvard Medical School and Massachusetts Eye and Ear

Stéphanie P. Lacour and Nicolas P. Grandjean, École Polytechnique Fédérale de Lausanne

Project Title: New generation of auditory brainstem implants
Project Summary:
The cochlear implant, a device that bypasses the deaf inner ear to convey electrical signals directly to the auditory nerve, has been the most successful neural prosthesis over the past few decades, with over 200,000 in use worldwide. However, some patients cannot receive an implant due to a damaged inner ear or auditory nerve. In their 2011 Bertarelli project, the researchers optimized design and fabrication of experimental auditory brainstem implants, using high‐density, flexible electrodes. Experiments were short-term, and only in mice. In this project, they will extend the research to long‐term experiments in mice to test the safety, durability, and effectiveness of the devices. They will also extend the flexible electrodes to human tissue, to optimize the geometry and stimulation parameters that will allow eventual use in human patients. 

PIs: Jeffrey R. Holt, Harvard Medical School and Boston Children's Hospital

Patrick Aebischer, École Polytechnique Fédérale de Lausanne

Project Title: Gene therapy to treat deafness
Project Summary:
For more than a decade hopes have been pinned on gene therapy to correct inherited disorders in humans. There are, for example, over 300 distinct inherited forms of deafness, which cause congenital deafness in about 1 in 1000 newborns, and these might be treated by gene therapy to replace defective genes. A longstanding problem for gene therapy for hearing loss, however, is that very few viral vectors will enter the mechanosensory cells of the inner ear.

This continuation of a successful project from 2011 will explore new vectors to carry genes into these sensory cells and will broaden the range of treatable genetic deafness. The researchers will also use modern genome editing technologies to repair specific mutations that cannot be corrected by simple gene replacement. 

PIs: Dara Manoach and Andre van der KouweHarvard Medical School and Massachusetts General Hospital

Dimitri Van De Ville, École Polytechnique Fédérale de Lausanne

Project Title: Brain networks in children with autism
Project Summary:
Functional magnetic resonance imaging (fMRI) has successfully allowed us to watch brain activity in humans during experimental tasks, revealing which brain regions are specialized for which computational functions. fMRI has also begun to be used to understand disorders of brain connectivity—the information flow between these regions. But movement of the head during imaging can distort the image, and children with autism tend to move more than others, impeding diagnosis.

These researchers will first develop methods to detect and correct for head motion in children and other difficult patients. They will then use fMRI scans of autistic children to test abnormal connectivity between brain regions, which is hypothesized as a cause of autism. Finally they will identify aspects of brain connectivity that correlate with specific types of autism, and ask whether connectivity can be improved with current autism treatments. These experiments will address general problems of fMRI in moving patients, with specific studies of autistic patients and the role of connectivity in this disorder. 

PIs: Michael J. Young, Harvard Medical School and Schepens Eye Research Institute

Matthias P. Lutolf and Yvan Arsenijevic, École Polytechnique Fédérale de Lausanne

Project Title: Tissue engineering the macula
Project Summary:
Retinal degenerative diseases are leading causes of incurable blindness and are often characterized by loss of the light-sensing photoreceptor cells. Because the regenerative capacity of the retina is extremely limited, cell transplantation strategies hold promise to restore lost function. Researchers have had some success in isolating the progenitor cells that can turn into photoreceptors, yet knowledge of the optimal stage of differentiation for transplantation is lacking.

The overall goal of this project is to develop cell lines that could be transplanted into the retina to reverse certain forms of blindness, and to discover drugs that could prevent or reverse retinal degeneration. This will be done in three stages: First, researchers will coax progenitor cells to become cone photoreceptors, the type of photoreceptor responsible for color vision and high-acuity vision. Second, researchers will engineer scaffolds that can support the growth and differentiation of these photoreceptors. The third stage is to use such scaffolds as a platform to test potential compounds that can reverse retinal degenerative disorders.